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Biochemistry

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JoVE Journal Biochemistry
Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
 

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Article DOI: 10.3791/58568-v • 11:31 min • November 30th, 2018
November 30th, 2018 •
*1, *1, *1,2,3,4, 2,3,4, 2,3,4, 1,2
1Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Tianjin University, 2State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology, Fujian Agriculture and Forestry University, 3Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, 4Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University
* These authors contributed equally

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In this article, we describe the protocols of protein expression, purification, crystallization and structure determination of the N-terminal domain of ryanodine receptor from diamondback moth (Plutella xylostella).

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Keywords: Ryanodine Receptor Crystal Structure Insecticide Insect Protein Structure X-ray Crystallography Protein Crystallography Polymerase Chain Reaction DNA Amplification LIC Vector T4 DNA Polymerase Ligation-independent Cloning BL21(DE3) E. Coli Protein Expression
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