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JoVE Journal Cancer Research
Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment
 

Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment

Article DOI: 10.3791/59507-v • 09:56 min • April 30th, 2019
April 30th, 2019 •
*1, *2,3, *1, 2,3, 1, 1, 2,3, 2,4,5,6, 1
1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College, Fudan University, 6Pancreatic Cancer Institute, Fudan University
* These authors contributed equally

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Automatic Translation

This protocol describes optimization procedures in a virus-based dual fluorescence-labeled tumor xenograft model using larval zebrafish as hosts. This heterogeneous xenograft model mimics the tissue composition of pancreatic cancer microenvironment in vivo and serves as a more precise tool for assessing drug responses in personalized zPDX (zebrafish patient-derived xenograft) models.

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Keywords: Zebrafish Larvae PDX Model Pancreatic Cancer Drug Assessment Fluorescent Labeling Cell Survival Personalized Medicine Xenograft In Vivo Tracing Tissue Dissociation Cell Preparation
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