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Developmental Biology

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JoVE Journal Developmental Biology
Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization
 

Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization

Article DOI: 10.3791/59916-v • 05:21 min • August 29th, 2019
August 29th, 2019 •
1, 1
1Graduate School of Horticulture, Chiba University

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We demonstrate a method to determine successful or failed fertilization on the basis of sperm nuclear morphology in Arabidopsis double fertilization using an epifluorescence microscope.

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Keywords: Arabidopsis Double Fertilization Sperm Nuclear Morphology Fertilization Evaluation HTR10-mRFP Emasculation Pollination Ovule Fluorescence Microscopy
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