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Bioengineering

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JoVE Journal Bioengineering
Biaxial Basal Tone and Passive Testing of the Murine Reproductive System Using a Pressure Myograph
 

Biaxial Basal Tone and Passive Testing of the Murine Reproductive System Using a Pressure Myograph

Article DOI: 10.3791/60125-v • 09:59 min • August 13th, 2019
August 13th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Biomedical Engineering, Tulane University

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This protocol utilized a commercially available pressure myograph system to perform pressure myograph testing on the murine vagina and cervix. Utilizing media with and without calcium, the contributions of the smooth muscle cells (SMC) basal tone and passive extracellular matrix (ECM) were isolated for the organs under estimated physiological conditions.

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Biaxial Basal Tone Passive Testing Murine Reproductive System Pressure Myograph Mechanical Properties Smooth Muscle Cells Passive Matrix Organ Geometry Native Smooth Muscle Cell Physiologic Range Of Pressures Axial Extensions Reproductive Pathologies Cervix Vagina Women's Health Biomechanics Blood Vessels Esophagus Large Intestines Suture The Tissue Unloaded Length Experimental Animal Absorbent Pad Dissecting Microscope Angled Tweezers Scissors
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