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Bioengineering

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JoVE Journal Bioengineering
Spatial Temporal Analysis of Fieldwise Flow in Microvasculature
 

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature

Article DOI: 10.3791/60493-v • 09:39 min • November 18th, 2019
November 18th, 2019 •
1,2, 1,3, 1, 4, 1,2, 5, 5, 5, 5, 6, 1,2, 5
1Biocomplexity Institute, Indiana University, 2Department of Intelligent Systems Engineering, Indiana University, 3Department of Physics, Indiana University, 4Scientific Designs, 5Department of Medicine, Indiana University, 6School of Public Health, Indiana University

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To quantify microvascular flow from high speed capillary flow image sequences, we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow) software. Across the full image field and over time, STAFF evaluates flow velocities and generates a sequence of color-coded spatial maps for visualization and tabular output for quantitative analyses.

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Keywords: Spatial Temporal Analysis Microvascular Flow Flow Velocities TrakEM2 Vascular Network Area List Paintbrush Tool Image Processing Fiji Brightness/contrast
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