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Journal / Genetics / Validating Whole Genome Nanopore Sequencing,…
JoVE Journal
Genetics

This Contenuto is Open Access.

JoVE Journal Genetics
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
 

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Article DOI: 10.3791/60906-v • 05:45 min • March 11th, 2020
March 11th, 2020 •
1, 1, 1, 1
1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research, Erasmus University Medical Center

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We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow cell.

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Artificial Intelligence AI Writing Assistants Copywriting Content Generation Text-based Content Content Creation AI-assisted Writing Writer's Block Content Efficiency Real-time Sequencing Virus Genetic Sequencing Nanopore Sequencing Usutu Virus Error Rate Read Coverage Sequence Data Consensus Sequence Reference-based Alignment Minimap2 Samtools KMA
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