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Biochemistry

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JoVE Journal Biochemistry
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
 

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Article DOI: 10.3791/61016-v • 08:31 min • April 10th, 2020
April 10th, 2020 •
*1, *1,2, 1, 1
1Department of Biochemistry and Molecular Biology, McGovern Medical School at UT Health, 2MD Anderson UT Health Graduate School
* These authors contributed equally

Capitoli

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Acyl-RAC (Acyl-Resin Assisted Capture) is a highly sensitive, reliable and easy to perform method to detect reversible lipid modification of cysteine residues (S-acylation) in a variety of biological samples.

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Protein S-acylation Acyl-resin Assisted Capture Acyl-RAC Detection Method Biological Samples Metabolic Labeling Radio Labeling Multiple Proteins Live Cells Primary Tissues Frozen Samples Cellular Processes Protein Trafficking Plasma Membrane Targeting Signal Transduction Iron Transport Protein-protein Interactions Hydroxylamine Solution Thioester Bond Chloroform Methanol Precipitation Sample Recovery Cell Lysates Centrifugation PBS Lysis Buffer
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