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Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
 

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Article DOI: 10.3791/61371-v • 05:46 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 3, 4, 1, 1, 1, For The Kidney Precision Medicine Project,
1Department of Medicine, Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology, Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine, Ohio State University Wexner Medical Center, 4Division of Pathology, Indiana University School of Medicine

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We describe a protocol for laser microdissection of sub-segments of the human kidney, including the glomerulus, proximal tubule, thick ascending limb, collecting duct and interstitium. The RNA is then isolated from the obtained compartments and RNA sequencing is carried out to determine changes in the transcriptomic signature within each sub-segment.

Tags

Laser Microdissection Transcriptomics Kidney Tissue Disease-induced Changes Omics Technologies Single Cell RNA Sequencing Cryostat Laser Microdissection Slide RNase-free 10%BSA FITC Phalloidin DAPI Alexa Fluor 546 RNase Inhibitor Acetone Humidity Chamber
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