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Kvantitativ Proteomics Workflow ved hjælp af flere reaktionsovervågningsbaserede detektioner af proteiner fra humant hjernevæv
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Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Kvantitativ Proteomics Workflow ved hjælp af flere reaktionsovervågningsbaserede detektioner af proteiner fra humant hjernevæv

DOI:
10.3791/61833-v

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11:49 min

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August 28, 2021

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, ,
1Centre for Research in Nanotechnology and Science,Indian Institute of Technology Bombay, 2Department of Biosciences and Bioengineering,Indian Institute of Technology Bombay

Chapters

  • 00:05Introduction
  • 00:49Protein Extraction from Brain Tissue
  • 02:04Protein Quantification and Quality Check
  • 02:32Protein Digestion
  • 03:58Desalting and Peptide Quantification
  • 05:06Transition List Preparation of Finalised Targets
  • 07:30LC Parameters
  • 08:03MS Parameters
  • 08:25Run Sequence and Instrument QC
  • 09:08Method Refinement
  • 10:58Results and Conclusions

Summary

Automatic Translation

Automatic Translation

Protokollen har til formål at indføre brugen af et tredobbelt firdobbelt massespektrometer til multireaktionsovervågning (MRM) af proteiner fra kliniske prøver. Vi har leveret en systematisk arbejdsgang fra prøveforberedelse til dataanalyse for kliniske prøver med alle de nødvendige forholdsregler, der skal træffes.

Tags

Quantitative ProteomicsMultiple Reaction MonitoringTargeted ProteomicsProtein DetectionSample PreparationProtein ExtractionProtein DigestionPeptide CleanupMass Spectrometry

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