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Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model
 

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

Article DOI: 10.3791/61902-v • 07:42 min • January 30th, 2021
January 30th, 2021 •
1, 1, 1,2
1Bioengineering Department, Temple University, 2Cancer Biology Program, Fox Chase Cancer Center

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Presented here is a protocol for the fabrication of a spheroid imaging device. This device enables dynamic or longitudinal fluorescence imaging of cancer cell spheroids. The protocol also offers a simple image processing procedure for the analysis of cancer cell invasion.

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Keywords: 3D Spheroid Model Cancer Cell Invasion Time-resolved Fluorescence Imaging Extracellular Matrix PDMS 3D Printing Spacer Glass Plates Vacuum Chamber Biopsy Punch Tissue Invasion Mammary Carcinoma
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