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Journal / Immunology and Infection / 使用免疫RNA-荧光原位杂交对SARS-CoV-2进行可视化…
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Immunology and Infection

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JoVE Journal Immunology and Infection
使用免疫RNA-荧光原位杂交对SARS-CoV-2进行可视化
 
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使用免疫RNA-荧光原位杂交对SARS-CoV-2进行可视化

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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在这里，我们描述了一个简单的方法，结合RNA荧光原位杂交（RNA-FISH）与免疫荧光可视化严重急性呼吸系统综合症冠状病毒2（SARS-CoV-2）RNA。该协议可以增加对SARS-CoV-2 RNA-主机在单细胞水平上的分子特征的理解。

Tags

免疫学和感染，第166期，SARS-CoV-2，RNA-FISH，杂交链反应，免疫荧光，人类气道上皮（HAE），渗透，共聚焦显微镜
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