Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation of Primary Rat Hepatocytes with…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
 

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Article DOI: 10.3791/62289-v • 07:35 min • April 5th, 2021
April 5th, 2021 •
*1, *2, 3, 4, 5, 1, 3, 1,3,4,5,6
1Department of Physiology & The Institute for Digital Medicine (WisDM), National University of Singapore, 2College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute, National University of Singapore, 4Institute of Bioengineering and Nanotechnology, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP, Singapore-MIT Alliance for Research and Technology
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

This protocol details the use of a special intravenous catheter, standardized sterile disposable tubing, temperature control complemented by real-time monitoring, and an alarm system for two-step collagenase perfusion procedure to improve the consistency in the viability, yield, and functionality of isolated primary rat hepatocytes.

Tags

Keywords: Primary Rat Hepatocytes Perfusion Control Liver Resection Collagenase Recirculation Portal Vein Cannulation Inferior Vena Cava Flow Rate Hepatocyte Isolation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter