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使用下一代测序来鉴定与CD4启动子附近CAS9诱导的双链断裂修复相关的突变

JoVE Journal
Genetica

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JoVE Journal Genetica

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

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使用下一代测序来鉴定与CD4启动子附近CAS9诱导的双链断裂修复相关的突变

DOI:

10.3791/62583-v

•

06:59 min

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March 31, 2022

•

Changkun Hu*¹, Tyler Doerksen*², Taylor Bugbee, Nicholas A. Wallace, Rachel Palinski³

¹Division of Biology,Kansas State University, ²Kansas State Veterinary Diagnostic Laboratory,Kansas State University, ³Department of Diagnostic Medicine/Pathobiology,Kansas State University

Capitoli

00:05Introduction
00:41Cell Plating
02:21Transfection
04:42Results: Identifying the Mutations Associated with Double Strand Break Repair near the CD4 Promoter
06:15Conclusion

Summary

Traduzione automatica

这里介绍的是sgRNA / CAS9内切酶和下一代测序方案，可用于鉴定与CD4启动子附近的双链断裂修复相关的突变。

Tags

Next Generation Sequencing CAS9 Double-strand Break CD4 Promoter Mutation Identification Human Keratinocytes Transfection CRISPR Gene Editing

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