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Immunology and Infection

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JoVE Journal Immunology and Infection
Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
 

Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model

Article DOI: 10.3791/63350-v • 05:41 min • April 6th, 2022
April 6th, 2022 •
1,2, 1, 2, 1
1Division of Gastroenterology, Department of Internal Medicine, University of Texas Medical Branch at Galveston, 2Department of Gastroenterology, Xiangya Hospital of Central South University

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The interaction of genetic susceptibility, mucosal immunity, and intestinal microecological environment is involved in the pathogenesis of inflammatory bowel disease (IBD). In this study, we applied fecal microbiota transplantation to IL-10 deficient mice and investigated its impact on colonic inflammation and heart function.

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Keywords: Fecal Microbiota Transplantation IL-10 Deficient Mouse Model Oral Gavage Sterile Technique Fecal Donor Fecal Suspension Homogenization Filtration Cryogenic Storage Thawing Gavage Needle
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