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Developmental Biology

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JoVE Journal Developmental Biology
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
 

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Article DOI: 10.3791/63799-v • 09:26 min • June 27th, 2022
June 27th, 2022 •
1, 2,3, 2,3, 2,3
1Department of Biology and Neuroscience Program, Hamline University, 2Department of Genetics, Cell Biology and Development, University of Minnesota, 3Developmental Biology Center, University of Minnesota

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This versatile protocol describes the isolation of premigratory neural crest cells (NCCs) through the excision of cranial neural folds from chick embryos. Upon plating and incubation, migratory NCCs emerge from neural fold explants, allowing for assessment of cell morphology and migration in a simplified 2D environment.

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Preparation Morphological Analysis Chick Cranial Neural Crest Cell Cultures Neural Crest Cells Cranial Neural Folds Primary Migratory Neural Crest Cells Cell Dissociation Two-dimensional Culture Visualization Investigation Migration-related Processes Cranial Neural Fold Culture Protocols Training Organism-specific Steps Dissection Plating Neural Folds Assessing Culture Outcomes Dissection Guidelines Morphometric Analysis Quantitative Results Fertilized Eggs Humidified Incubator Disinfecting Shells 70% Ethanol Fixing And Staining Cultures Glass Cover Slips Multi-well Tissue Culture Dish Fibronectin Solution
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