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Journal / Medicine / Rectal Organoid Morphology Analysis (ROMA): A…
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JoVE Journal Medicine
Rectal Organoid Morphology Analysis (ROMA)
 

Rectal Organoid Morphology Analysis (ROMA): A Diagnostic Assay in Cystic Fibrosis

Article DOI: 10.3791/63818-v • 07:56 min • June 10th, 2022
June 10th, 2022 •
1,2, 1, 3,4, 5, 6, 7,8, 7,9, 8, 3,4, 1,2, 1,2, 10,11, 1,2, 1,2
1Department of Development and Regeneration, Woman and Child Unit, CF research lab, KU Leuven, 2Department of Pediatrics, Pediatric Pulmonology, University Hospitals Leuven, 3VIB Bio Imaging Core, VIB-KU Leuven Center for Brain & Disease Research, 4Department for Neuroscience, KU Leuven, 5Interuniversity Center for Biostatistics and Statistical Bioinformatics, University of Leuven and University of Hasselt, 6Department of Pediatrics, 2nd Faculty of Medicine, Charles University and Motol University Hospital, 7Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, 8Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL), KU Leuven, 9Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, 10Department of Chronic Diseases, Metabolism and Ageing; Pneumology, KU Leuven, 11Department of Respiratory Diseases, University Hospitals Leuven

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Automatic Translation

This protocol describes rectal organoid morphology analysis (ROMA), a novel diagnostic assay for cystic fibrosis (CF). Morphological characteristics, namely the roundness (circularity index, CI) and the presence of a lumen (intensity ratio, IR), are a measure of CFTR function. Analysis of 189 subjects showed perfect discrimination between CF and non-CF.

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Keywords: Rectal Organoid ROMA Cystic Fibrosis CFTR Mutations Sweat Fluoride Semi-automated Standardized CFTR Modulatory Efficacy Calcium Green Live Cell Imaging Image Analysis Exclusion Criteria Plating Density Fluorescence Distribution Image Recalibration Structure Delineation Image Segmentation
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