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An Integrated Approach for Microprotein Identification and Sequence Analysis
 

An Integrated Approach for Microprotein Identification and Sequence Analysis

Article DOI: 10.3791/63841-v • 09:37 min • July 12th, 2022
July 12th, 2022 •
*1, *1, 1,2
1The Heart Institute, Division of Molecular Cardiovascular Biology, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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The protocol described here provides detailed instructions on how to analyze genomic regions of interest for microprotein-coding potential using PhyloCSF on the user-friendly UCSC Genome Browser. Additionally, several tools and resources are recommended to further investigate sequence characteristics of identified microproteins to gain insight into their putative functions.

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Keywords: Microprotein Identification Sequence Analysis PhyloCSF UCSC Genome Browser Non-coding Regions Protein-coding Potential Comparative Genomics Bioinformatics Blat Genome Assembly DNA Sequence Protein Sequence
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