DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Changyue Liu; Zhengyang Lei; Lijin Lian; Likun Zhang; Zhicheng Du; Peiwu Qin

doi:10.3791/64833

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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Article DOI: 10.3791/64833-v • 04:17 min • May 10th, 2024

May 10th, 2024 •

Changyue Liu*^1,2, Zhengyang Lei*^1,2, Lijin Lian², Likun Zhang^1,2, Zhicheng Du^1,2, Peiwu Qin^1,2

¹Center of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, ²Institute of Biopharmaceutics and Health Engineering, Tsinghua Shenzhen International Graduate School

* These authors contributed equally

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We present a protocol that combines recombinase polymerase amplification with a CRISPR/Cas12a system for trace detection of DNA viruses and builds portable smartphone microscopy with an artificial intelligence-assisted classification for point-of-care DNA virus detection.

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

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