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在体内的器官型小鼠视网膜Wholemount文化
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JoVE 신문 생물학
In vivo-like Organotypic Murine Retinal Wholemount Culture
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在体内的器官型小鼠视网膜Wholemount文化

DOI:
10.3791/1634-v

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25:26 min

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January 11, 2010

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,
1Institute for Anatomy, Department of Neuroanatomy,University of Duisburg-Essen

Chapters

  • 00:00Title
  • 00:35Introduction
  • 01:50Enucleation of Murine Eyes and Dissection of Retinas
  • 04:02Dissection of Embryonic, Postnatal and Adult Murine Retinas
  • 11:40Murine Organotypic Retinal Wholemount Culture
  • 14:10Dissociation of Cultured Retinal Wholemounts
  • 19:35Washing of Dissociated Cell Suspensions
  • 24:49Conclusion

Summary

자동 번역

자동 번역

此视频文章演示器官视网膜wholemount文化和cytospin一个外生的诱导效应分析程序的建立。器官型视网膜wholemount文化模仿<em>在体内</em>情况，同时规避经典的小鼠动物模型的缺点，大大促进了实验操作的小鼠视网膜的无障碍。

Tags

In Vivo-like Organotypic Murine Retinal Wholemount CultureTargeted Gene AblationsAnimal ModelsRetinal Gene FunctionTransgenic Mouse ModelsConditional Knockout Mouse ModelsPrimary Cell CultureRecombinant FactorsGene OverexpressionSiRNA-mediated Gene KnockdownControlled EnvironmentDissociated Cell CultureEndogenous SignalsExogenously Triggered EffectsPharmacological ManipulationOrganotypic Retinal Wholemount CulturesPhysiological In Vivo SituationNeuronal InteractionsConnections PreservedDissection PeculiaritiesEmbryonic EyesPostnatal EyesAdult Murine EyesNeuronal ApoptosisRetinal Cell Proliferation

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