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Chapters
- 00:00Title
- 00:35Introduction
- 01:50Enucleation of Murine Eyes and Dissection of Retinas
- 04:02Dissection of Embryonic, Postnatal and Adult Murine Retinas
- 11:40Murine Organotypic Retinal Wholemount Culture
- 14:10Dissociation of Cultured Retinal Wholemounts
- 19:35Washing of Dissociated Cell Suspensions
- 24:49Conclusion
Deze video artikel demonstreert de oprichting van organotypische netvlies wholemount culturen en een Cytospin procedure voor de analyse van exogeen geïnduceerde effecten. Organotypische netvlies wholemount culturen na te bootsen van de<em> In vivo</em> Situatie en aanzienlijk vergemakkelijken de toegankelijkheid van muriene retina's voor experimentele manipulaties, terwijl het omzeilen van de nadelen van de klassieke murine diermodellen.
Tags
In Vivo-like Organotypic Murine Retinal Wholemount CultureTargeted Gene AblationsAnimal ModelsRetinal Gene FunctionTransgenic Mouse ModelsConditional Knockout Mouse ModelsPrimary Cell CultureRecombinant FactorsGene OverexpressionSiRNA-mediated Gene KnockdownControlled EnvironmentDissociated Cell CultureEndogenous SignalsExogenously Triggered EffectsPharmacological ManipulationOrganotypic Retinal Wholemount CulturesPhysiological In Vivo SituationNeuronal InteractionsConnections PreservedDissection PeculiaritiesEmbryonic EyesPostnatal EyesAdult Murine EyesNeuronal ApoptosisRetinal Cell Proliferation
