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Chapters
- 00:00Title
- 00:35Introduction
- 01:50Enucleation of Murine Eyes and Dissection of Retinas
- 04:02Dissection of Embryonic, Postnatal and Adult Murine Retinas
- 11:40Murine Organotypic Retinal Wholemount Culture
- 14:10Dissociation of Cultured Retinal Wholemounts
- 19:35Washing of Dissociated Cell Suspensions
- 24:49Conclusion
Dieses Video Artikel veranschaulicht die Einrichtung von organotypischen Retina wholemount Kulturen und einer Cytospin Verfahren zur Analyse von exogen induzierten Effekte. Organotypischen Retina wholemount Kulturen imitieren die<em> In vivo</em> Situation und deutlich erleichtern die Zugänglichkeit von murinen Retina für experimentelle Manipulationen unter Umgehung der Nachteile der klassischen Maus-Tiermodell.
Tags
In Vivo-like Organotypic Murine Retinal Wholemount CultureTargeted Gene AblationsAnimal ModelsRetinal Gene FunctionTransgenic Mouse ModelsConditional Knockout Mouse ModelsPrimary Cell CultureRecombinant FactorsGene OverexpressionSiRNA-mediated Gene KnockdownControlled EnvironmentDissociated Cell CultureEndogenous SignalsExogenously Triggered EffectsPharmacological ManipulationOrganotypic Retinal Wholemount CulturesPhysiological In Vivo SituationNeuronal InteractionsConnections PreservedDissection PeculiaritiesEmbryonic EyesPostnatal EyesAdult Murine EyesNeuronal ApoptosisRetinal Cell Proliferation
