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JoVE Journal Biology
Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
 

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Article DOI: 10.3791/50316-v • 15:55 min • June 21st, 2013
June 21st, 2013 •
*1, *1,2, 1,2
1Department of Pharmacology, Rush University, 2Pulmonary and Critical Care Department, Rush University
* These authors contributed equally

챕터

요약
Automatic Translation

Repeated retro-orbital injections of cationic liposomes/siRNA/ITSN-1s complexes in mice, every 72 hours for 24 days, efficiently deliver the siRNA duplex to the mouse lung microvasculature reducing ITSN-1s mRNA and protein expression by 75%. This technique is highly reproducible in an animal model, has no adverse effects and avoids fatalities.

Tags

Keywords: SiRNA ITSN-1s Cationic Liposomes Lung Endothelium Electron Microscopy Gene Silencing Mouse Model Vascular Permeability Endothelial Cell Survival
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