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Journal / Immunology and Infection / 激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量…
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Immunology and Infection

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JoVE Journal Immunology and Infection
激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量
 
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激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量

Article DOI: 10.3791/51284-v • 09:04 min • May 22nd, 2014
May 22nd, 2014 •
1, 2, 3
1Department of Pathology, New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center

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Click here for the English version.

树突状细胞（DC）分泌IL-1β响应于TLR8识别合成嘌呤，R848，接着NLRP3炎性体激活与尼日利亚菌素，因此，IL-1β可以用来测量NLRP3炎性活性。细胞内细胞因子染色法，免疫印迹和ELISA法用于准确地测量通过IL-1β表达NLRP3炎性引发和活化。

Tags

免疫学，第87，NLRP3，炎性，IL-1β，白细胞介素-1β，树突状细胞，尼日利亚菌素，Toll样受体8，TLR8，R848，单核细胞来源的树突状细胞
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