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Journal / Immunology and Infection / 炎症性半月酶诱导的人单核细胞来源巨噬细胞邻近性的可视化…
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Immunology and Infection

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JoVE Journal Immunology and Infection
炎症性半月酶诱导的人单核细胞来源巨噬细胞邻近性的可视化
 
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炎症性半月酶诱导的人单核细胞来源巨噬细胞邻近性的可视化

Article DOI: 10.3791/63162-v • 08:41 min • April 6th, 2022
April 6th, 2022 •
1, 1
1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology, Baylor College of Medicine

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该协议描述了从人血液样本中获取单核细胞来源巨噬细胞（MDM）的工作流程，一种在不损害细胞活力和行为的情况下将炎症半胱天冬酶双分子荧光互补（BiFC）报告基因有效引入人MDM的简单方法，以及一种基于成像的方法来测量活细胞中的炎症半胱天冬酶活化。

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免疫学与感染，第182期，
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