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JoVE Journal Chemistry
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
 

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

Article DOI: 10.3791/51441-v • 10:56 min • May 30th, 2014
May 30th, 2014 •
1, 1, 1,2, 1
1Department of Chemical and Materials Engineering, University of Alberta, 2Department of Chemical and Petroleum Engineering, University of Calgary

챕터

요약
Automatic Translation

A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.

Tags

Keywords: Algal Cells Neutral Lipids Fluorescence Nile Red Auxenochlorella Protothecoides Lipid Content Bioprocess Monitoring Rapid Protocol Gravimetric Methods Cell Membrane Permeabilization
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