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Analyse van de epitheelbeschadiging Geproduceerd door<em> Entamoeba histolytica</em> Infectie
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1Department of Infectomics and Molecular Pathogenesis,Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Molecular Biomedicine,Center for Research and Advanced Studies of the National Polytechnic Institute, 3Agency for Knowledge Commercialization,Center for Research and Advanced Studies of the National Polytechnic Institute
Chapters
- 00:05Title
- 02:43Preparation of Cultures and Trophozoite Lysates
- 04:00Preparation of Trophozoite Secreted Products
- 04:58Interaction of MDCK Cells with Trophozoites, Trophozoite Lysates or Secreted Products
- 07:33Measurement of Transepithelial Electrical Resistance (TER)
- 08:42Results: Epithelial Damage by Entamoeba histolytica
- 10:27Conclusion
Menselijke infectie door Entamoeba histolytica leidt tot amoebiasis, een belangrijke oorzaak van diarree in tropische landen. Infectie wordt gestart door pathogeen interactie met intestinale epitheelcellen, veroorzaken de opening van cel-cel contacten en bijgevolg diarree, soms gevolgd door leverbesmetting. Dit artikel geeft een model om de vroege gastheer-pathogeen interacties beoordelen in ons begrip van amoebiasis pathogenese verbeteren.
