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JoVE Journal Medicine
Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs
 

Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs

Article DOI: 10.3791/54077-v • 10:47 min • July 2nd, 2016
July 2nd, 2016 •
1,2, 1, 1, 1, 1, 1, 1, 1
1College of Veterinary Medicine, Gyeongsang National University, 2PWG Genetics Pte Ltd.

챕터

요약
Automatic Translation

A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.

Tags

Keywords: Mesenchymal Stem Cells Synovial Fluid Bone Marrow Minipigs Regeneration Immunomodulation Joint Diseases Autologous Bone Marrow Extraction Density Centrifugation Mononuclear Cells
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