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Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli
 

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

Article DOI: 10.3791/54294-v • 13:10 min • September 25th, 2016
September 25th, 2016 •
1, 1, 1, 2, 2, 3, 2, 4
1Molecular Imaging and Neurovascular Research Laboratory, Dongguk University College of Medicine, 2Biomedical Research Center, Korea Institute of Science and Technology, 3Research Institute of Advanced Materials, Department of Materials Science and Engineering, Seoul National University, 4Departments of Radiology and Cancer Systems Imaging, University of Texas M.D. Anderson Cancer Center

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요약
Automatic Translation

This protocol describes the application of combined near-infrared fluorescent (NIRF) imaging and micro-computed tomography (microCT) for visualizing cerebral thromboemboli. This technique allows the quantification of thrombus burden and evolution. The NIRF imaging technique visualizes fluorescently labeled thrombus in excised brain, while the microCT technique visualizes thrombus inside living animals using gold-nanoparticles.

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Keywords: Cerebral Thromboembolism Near-infrared Fluorescent Imaging Micro-computed Tomography Gold Nanoparticles Thrombolytic Agents Tissue Plasminogen Activator Hemostasis Thrombosis In Vivo Ex Vivo Micro-CT Fluorescent Thrombus Imaging Cardiac Puncture Fibrin Cross-linking Polyethylene Tube PBS PE10 Tube PE50 Tube Laser Doppler Flow Meter
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