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JoVE Journal Genetics
Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs
 

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

Article DOI: 10.3791/55239-v • 11:24 min • June 12th, 2017
June 12th, 2017 •
1, 2, 3, 1
1Toxicology and Mycotoxin Research Unit, NPRC, USDA-ARS, 2Hazera Seeds LTD, Brurim, 3Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” - Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”

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요약
Automatic Translation

Gene deletion mutants generated through homologous recombination are the gold standard for gene function studies. The OSCAR (One Step Construction of Agrobacterium-Recombination-ready-plasmids) method for rapid generation of deletion constructs is described. Agrobacterium mediated fungal transformation follows. Finally, a PCR based confirmation method of gene deletions in fungal transformants is presented.

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Keywords: Rapid Deletion Production Fungi Agrobacterium OSCAR Deletion Constructs Homologous Recombination Functional Genetics Gene Deletion Mutants DelsGate System Primer Design PCR Deletion Confirmation
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