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JoVE Journal
Genetics

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Chromatine Immunoprecipitatie (ChIP) in Muis T-cellijnen
 
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Chromatine Immunoprecipitatie (ChIP) in Muis T-cellijnen

Article DOI: 10.3791/55907-v 11:39 min June 17th, 2017
June 17th, 2017

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Dit werk beschrijft een protocol voor chromatine immunoprecipitatie (ChIP) met behulp van een volwassen T-cellijn van de muis. Dit protocol is geschikt om de verdeling van specifieke histonenmerken op specifieke promotor sites of genoombreed te onderzoeken.

Tags

Genetica Uitgave 124 ChIP H3K4me1 H3K4me3 H3K27ac H3 histonenmerken sonicatie scheren chromatine regulering transcriptie.
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