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Immunology and Infection

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JoVE Journal Immunology and Infection
Multiplexed fluorescerende immunohistochemische kleuring, Imaging en analyse in histologische monsters van lymfoom
 
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Multiplexed fluorescerende immunohistochemische kleuring, Imaging en analyse in histologische monsters van lymfoom

Article DOI: 10.3791/58711-v • 07:52 min • January 9th, 2019
January 9th, 2019 •
*1,2, *3, 3, 2, 2, 2, 4, 4, 2,3, 2,4
1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System
* These authors contributed equally

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Hier beschrijven we een protocol voor multiplex fluorescerende immunohistochemische kleuring en imaging voor de gelijktijdige localisatie van meerdere kanker-geassocieerde antigenen in lymfoom. Dit protocol kan worden uitgebreid tot de colocalization analyse van biomarkers binnen alle weefselsecties.

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Immunologie en infecties probleem 143 gemultiplext immunofluorescentie multispectrale imaging kwantitatieve analyse biomarkers lymfoom digitale pathologie
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