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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
 

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Article DOI: 10.3791/59734-v • 12:15 min • May 29th, 2019
May 29th, 2019 •
*1,2, *1, 3, 3, 3,4, 5, 5, 1
1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 2Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, 4Instituto de Estudos Avançados, Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo
* These authors contributed equally

챕터

요약
Automatic Translation

We describe here methods for sensitive and accurate quantification of the lesions 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 1,N6-etheno-2'-deoxyadenosine (1,N6-dAdo) and 1,N2-etheno-2'-deoxyguanosine (1,N2-dGuo) in DNA. The methods were applied to the assessment of the effects of ambient fine particulate matter (PM2.5) in tissues (lung, liver and kidney) of exposed A/J mice.

Tags

DNA Lesions Mass Spectrometry Quantification Oxidative Stress Tissues Mice Ambient Fine Particulate Matter Selectivity Sensitivity HPLC Tandem Mass Spectrometry Etheno Adducts Inflammatory Diseases Pollutants Cancer Development Sample Preparation Culture Plate Tissue Scalpel Weigh
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