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Journal / Developmental Biology / Isolation, Kultur og adipogen induktion af…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolation, Kultur og adipogen induktion af neuralcrest original fedt-afledte stamceller fra periaortisk fedtvæv
 
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Isolation, Kultur og adipogen induktion af neuralcrest original fedt-afledte stamceller fra periaortisk fedtvæv

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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요약
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Vi præsenterer en protokol for isolation, kultur og adipogen induktion af neurale crest afledte stamceller (NCADSCs) fra det periaortiske fedtvæv af Wnt-1 Cre+/-; Rosa26RFP/+ mus. NCADSCs kan være en let tilgængelig kilde til Adscs til modellering adipogenesis eller lipogenese in vitro.

Tags

Udviklingsmæssige Biologi neurale crest celle Wnt-1 mus periaortisk fedtvæv fedt-afledte stromale celler stromale vaskulære fraktion cellekultur adipogenic induktion
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