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Developmental Biology

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JoVE Journal Developmental Biology
Isolatie, cultuur en adipogene inductie van neurale crest originele vet-afgeleide stamcellen uit periaortic vetweefsel
 
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Isolatie, cultuur en adipogene inductie van neurale crest originele vet-afgeleide stamcellen uit periaortic vetweefsel

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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We presenteren een protocol voor de isolatie, cultuur en adipogene inductie van neurale kam afgeleide vet-afgeleide stamcellen (NCADSCs) uit de periaortische vetweefsel van Wnt-1 Cre+/-; Rosa26RFP/+ muizen. De NCADSCs kunnen een gemakkelijk toegankelijke bron van ADSCs voor het modelleren van adipogenese of lipogenese in vitro.

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Ontwikkelingsbiologie Kwestie 157 neurale kamcel Wnt-1 muis periaortisch vetweefsel vet-afgeleide stromalcellen stromal vasculaire fractie celcultuur adipogene inductie
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