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Journal / Developmental Biology / 神経紋の分離、培養、脂肪形成誘導、周食脂肪組織由来の脂肪由来幹細胞…
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JoVE Journal Developmental Biology
神経紋の分離、培養、脂肪形成誘導、周食脂肪組織由来の脂肪由来幹細胞
 
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神経紋の分離、培養、脂肪形成誘導、周食脂肪組織由来の脂肪由来幹細胞

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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Click here for the English version.

Wnt-1 Cre+/-の周産脂肪組織から誘導された脂肪由来幹細胞 (NCAC) の神経堤の分離、培養、脂肪誘導のプロトコルを提示します。ローザ26RFP/+マウス。NCAdscは、インビトロで脂肪形成または脂肪形成をモデル化するためのACの簡単にアクセス可能なソースであることができます。

Tags

発生生物学,問題157,神経堤細胞,Wnt-1,マウス,帯皮脂肪組織,脂肪由来間質細胞,間質血管画分,細胞培養,脂肪形成誘導
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