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子结构分析仪:用于荧光显微镜图像中细胞体快速探索和精确分析的用户友好型工作流程
JoVE 신문
생체공학
Author Produced
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JoVE 신문 생체공학
Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
DOI:

14:28 min

July 15, 2020

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Chapters

  • 00:00Introduction
  • 01:06Download Icy and the “Substructure Analyzer” Protocol on Icy Website
  • 01:30Opening the Protocol
  • 02:03Interacting with the Workflow on Icy
  • 02:49Merging of the Channels of an Image
  • 04:57Segmentation of the Regions of Interest
  • 07:42Fluorescent Signal Detection and Analysis
  • 09:25Run the Protocol
  • 10:35Representative Results
  • 13:48Conclusion

Summary

자동 번역

我们提出了一个自由可用的工作流程,用于在荧光显微镜图像中快速探索和准确分析特定细胞腔中的细胞体。此用户友好的工作流在开源软件 Icy 上设计,并且还使用 ImageJ 功能。在没有图像分析知识的情况下,管道是负担得起的。

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