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Immunology and Infection
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JoVE Journal Immunology and Infection
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
 
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Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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Nous présentons un essai RT-LAMP pour la détection de TiLV chez le poisson tilapia à l’aide d’instruments simples sur une période de temps relativement courte par rapport aux techniques RT-PCR conventionnelles. Ce protocole peut aider à contrôler la propagation épidémique de TiLVD, en particulier dans les pays en développement.

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Immunologie et infection numéro 159 virus du lac tilapia diagnostic tilapia RT-LAMP RT-qPCR outil de dépistage
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