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Immunology and Infection
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JoVE Journal Immunology and Infection
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay für die spezifische und schnelle Detektion von Tilapia Lake Virus
 
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Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay für die spezifische und schnelle Detektion von Tilapia Lake Virus

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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Wir präsentieren einen RT-LAMP-Test für den Nachweis von TiLV in Tilapia-Fischen mit einfachen Instrumenten über einen relativ kurzen Zeitraum im Vergleich zu herkömmlichen RT-PCR-Techniken. Dieses Protokoll kann dazu beitragen, die epidemische Ausbreitung von TiLVD, insbesondere in Entwicklungsländern, zu kontrollieren.

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Immunologie und Infektion Ausgabe 159 Tilapia-Seevirus Diagnose Tilapia RT-LAMP RT-qPCR Screening-Tool
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