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JoVE Journal Neuroscience
In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers om Cortical Mouse Astrocytes te individualiseren
 
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In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers om Cortical Mouse Astrocytes te individualiseren

Article DOI: 10.3791/61110-v • 06:47 min • May 21st, 2020
May 21st, 2020 •
*1, *2, 1, 3, 4, 2, 1, 4, 3, 2, 1
1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris
* These authors contributed equally

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Astrocyten betegelen de hersenschors uniform, waardoor de analyse van hun complexe morfologie uitdagend is op cellulair niveau. Het protocol dat hier wordt verstrekt, maakt gebruik van multicolor-etikettering op basis van in utero-elektroporatie om corticale astrocyten uit te schakelen en hun volume en morfologie te analyseren met een gebruiksvriendelijke pijplijn voor beeldanalyse.

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Neurowetenschappen hersenschors astrocyten ontwikkeling celvolume morfologie transgenen elektroporatie genoverdrachtstechnieken microscopie
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