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Immunology and Infection

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JoVE Journal Immunology and Infection
Etablering af en Porcine Ex Vivo Hornhinde Model for at studere medicinske behandlinger mod bakteriel Keratitis
 
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Etablering af en Porcine Ex Vivo Hornhinde Model for at studere medicinske behandlinger mod bakteriel Keratitis

Article DOI: 10.3791/61156-v • 09:22 min • May 12th, 2020
May 12th, 2020 •
1,2, 3, 4, 1,5, 3, 1,6, 1,7, 1,2
1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB), University of Sheffield, 2Department of Chemical and Biological Engineering, University of Sheffield, 3Hyderabad Eye Research Foundation, L V Prasad Eye Institute, 4Health Economics and Decision Science, School of Health and Related Research, University of Sheffield, 5Department of Molecular Biology and Biotechnology, University of Sheffield, 6Department of Materials Science and Engineering, University of Sheffield, 7Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield

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I denne artikel beskrives en trinvis protokol til opsætning af en ex vivo-svinemodel af bakteriel keratitis. Pseudomonas aeruginosa bruges som en prototypisk organisme. Denne innovative model efterligner in vivo infektion som bakteriel spredning er afhængig af bakteriens evne til at beskadige hornhindevæv.

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Immunologi og infektion Problem 159 Pseudomonas aeruginosa keratitis hornhinde ex vivo øje svin
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