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Journal / Immunology and Infection / Établissement d’un modèle de cornée Porcine Ex…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Établissement d’un modèle de cornée Porcine Ex Vivo pour l’étude des traitements médicamenteux contre la kératite bactérienne
 
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Établissement d’un modèle de cornée Porcine Ex Vivo pour l’étude des traitements médicamenteux contre la kératite bactérienne

Article DOI: 10.3791/61156-v • 09:22 min • May 12th, 2020
May 12th, 2020 •
1,2, 3, 4, 1,5, 3, 1,6, 1,7, 1,2
1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB), University of Sheffield, 2Department of Chemical and Biological Engineering, University of Sheffield, 3Hyderabad Eye Research Foundation, L V Prasad Eye Institute, 4Health Economics and Decision Science, School of Health and Related Research, University of Sheffield, 5Department of Molecular Biology and Biotechnology, University of Sheffield, 6Department of Materials Science and Engineering, University of Sheffield, 7Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield

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Cet article décrit un protocole étape par étape pour mettre en place un modèle ex vivo porcin de kératite bactérienne. Pseudomonas aeruginosa est utilisé comme un organisme prototypique. Ce modèle novateur imite l’infection in vivo car la prolifération bactérienne dépend de la capacité de la bactérie à endommager le tissu cornéen.

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Immunologie et infection Numéro 159 Pseudomonas aeruginosa kératite cornée ex vivo oeil porcine
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