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使用自动成像系统测量 iPSC 的汇合度。

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Measuring the Confluence of iPSCs Using an Automated Imaging System

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使用自动成像系统测量 iPSC 的汇合度。

DOI:

10.3791/61225-v

•

11:39 min

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June 10, 2020

•

Valentina Magliocca², Maria Vinci, Tiziana Persichini, Franco Locatelli, Marco Tartaglia, Claudia Compagnucci

¹Genetics and Rare Diseases Research Division,Ospedale Pediatrico Bambino Gesù, IRCCS, ²Department of Science,University Roma Tre, ³Department of Onco-hematology, Gene and Cell Therapy,Ospedale Pediatrico Bambino Gesù, IRCCS

Chapters

00:04Introduction
00:4296-Well Plate Coating
02:49Induced Pluripotent Stem Cell (iPSC) Culture Maintenance
05:03Cell Confluence Characterization
06:53Cytoskeletal Microfilament Characterization
09:02Results: Representative Analyses of Cell Confluence on Differently Coated Vessels
10:57Conclusion

Summary

자동 번역

该方案的目标是比较不同的细胞外基质（ECM）包被条件，以评估差异涂层如何影响诱导多能干细胞（iPSC）的生长速率。特别是，我们的目标是创造条件以获得iPSC培养物的最佳生长。

Tags

IPSCs Induced Pluripotent Stem Cells Automated Imaging System Confluence Extracellular Matrix Basement Membrane Matrix Laminin Vitronectin Fibronectin Mouse Embryonic Fibroblasts Cell Culture Cryopreservation

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