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JoVE Journal Cancer Research
En automatiseret mikroskopisk scoringsmetode til γ-H2AX Foci-analysen i humane perifere blodlymfocytter
 
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En automatiseret mikroskopisk scoringsmetode til γ-H2AX Foci-analysen i humane perifere blodlymfocytter

Article DOI: 10.3791/62623-v • 08:23 min • December 25th, 2021
December 25th, 2021 •
1, 2, 1, 3, 1, 1, 1, 1, 4, 5, 6, 1
1Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, 2Department of Biotechnology, University of the Western Cape, 3Department of Medical Biosciences, University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, 6MetaSystems Hard & Software GmbH

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Denne protokol præsenterer dias forberedelse og automatiseret scoring af γ-H2AX foci assay på perifere blod lymfocytter. For at illustrere analysens metode og følsomhed blev isolerede lymfocytter bestrålet in vitro. Denne automatiserede metode til DNA DSB-detektion er nyttig til hurtige og højgennemsigtede biologiske dosimetryapplikationer.

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