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Isolation of Human Neutrophils from Whole Blood and Buffy Coats
 

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Article DOI: 10.3791/62837-v • 08:12 min • September 17th, 2021
September 17th, 2021 •
1, 1, 1, 1,2
1Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science, Mahidol University

챕터

요약
Automatic Translation

This protocol details a method for the isolation of neutrophils from whole blood, buffy coats, or leukapheresis membranes, achieving good yield, high purity, and minimal cell activation. We utilize gradient purification, red blood cell (RBC) sedimentation, and RBC lysis to obtain a high-quality/purity neutrophil preparation.

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Keywords: Neutrophils Density Gradient Sedimentation Lysis Buffy Coat PBMC Erythrocyte Cell Biology Cell Isolation Cell Preparation
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