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Biochemistry

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JoVE Journal Biochemistry
Uracil-DNA-glycosylaseassay ved matrixassisteret laserdesorption/ionisering Time-of-flight Massespektrometrianalyse
 
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Uracil-DNA-glycosylaseassay ved matrixassisteret laserdesorption/ionisering Time-of-flight Massespektrometrianalyse

Article DOI: 10.3791/63089-v • 12:19 min • April 22nd, 2022
April 22nd, 2022 •
*1, *1,2, 3, 2, 1,2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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En ikke-mærket, ikke-radio-isotopisk metode til assay uracil-DNA-glycosylaseaktivitet blev udviklet ved anvendelse af MALDI-TOF massespektrometri til direkte apurinisk/ apyrimidinisk stedholdig produktanalyse. Assayet viste sig at være ret simpelt, specifikt, hurtigt og let at bruge til DNA-glycosylasemåling.

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Biokemi udgave 182 Reparation af basisudskæring MALDI-TOF massespektrometri Uracil-DNA-glycosylase apurinisk/apyrimidinisk sted glycosylasehæmmer
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