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JoVE Journal Bioengineering
Autofluorescence Imaging to Evaluate Cellular Metabolism
 

Autofluorescence Imaging to Evaluate Cellular Metabolism

Article DOI: 10.3791/63282-v • 07:36 min • November 15th, 2021
November 15th, 2021 •
1, 1, 1, 1, 1
1Department of Biomedical Engineering, Texas A&M University-College Station

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This protocol describes fluorescence imaging and analysis of the endogenous metabolic coenzymes, reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H), and oxidized flavin adenine dinucleotide (FAD). Autofluorescence imaging of NAD(P)H and FAD provides a label-free, nondestructive method to assess cellular metabolism.

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Keywords: Autofluorescence NADH FAD Cellular Metabolism Multi-photon Fluorescence Lifetime Microscopy Live Cell Imaging Non-destructive
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