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Journal / Bioengineering / 自发荧光成像评估细胞代谢
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JoVE Journal Bioengineering
自发荧光成像评估细胞代谢
 
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自发荧光成像评估细胞代谢

Article DOI: 10.3791/63282-v • 07:36 min • November 15th, 2021
November 15th, 2021 •
1, 1, 1, 1, 1
1Department of Biomedical Engineering, Texas A&M University-College Station

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该协议描述了内源性代谢辅酶，还原烟酰胺腺嘌呤（磷酸盐）二核苷酸（NAD（P）H）和氧化黄素腺嘌呤二核苷酸（FAD）的荧光成像和分析。NAD（P）H 和 FAD 的自发荧光成像提供了一种无标记、无损的方法来评估细胞代谢。

Tags

生物工程，第177期，FLIM，代谢，NAD（P）H，FAD，荧光，显微镜
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