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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
 

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Article DOI: 10.3791/63704-v 10:40 min April 25th, 2022
April 25th, 2022

챕터

요약

This protocol describes a high-throughput clustered regularly interspaced short palindromic repeats (CRISPR) gene editing workflow for microRNA cluster network analysis that allows the rapid generation of a panel of genetically modified cell lines carrying unique miRNA cluster member deletion combinations as large as 35 kb within a single experiment.

Tags

Keywords: CRISPR Gene Editing MicroRNA Cluster Network Analysis High-throughput Cancer Pathways Tumor Growth Drug Resistance Genomic Sequence CRISPR RNA Genotyping Cas9 Doxycycline Western Blot
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