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Journal / Biochemistry / 使用蛋白质印迹法研究神经元K-Cl共转运蛋白KCC2的功能和活性…
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JoVE Journal Biochemistry
使用蛋白质印迹法研究神经元K-Cl共转运蛋白KCC2的功能和活性
 
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使用蛋白质印迹法研究神经元K-Cl共转运蛋白KCC2的功能和活性

Article DOI: 10.3791/64179-v • 10:08 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1
1Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences, University of Exeter, Hatherly Laboratories

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本协议重点介绍了蛋白质印迹技术在研究神经元K-Cl共转运蛋白KCC2的功能和活性方面的应用。该方案描述了通过蛋白质印迹 在 激酶调节位点Thr906/1007处KCC2磷酸化的研究。此外，本文还简要强调了确认KCC2活性的其他方法。

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生物化学，第190期，
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