David Lillicrap Department of Pathology and Molecular Medicine Queen's University Biography Publications Institution JoVE Articles David Lillicrap has not added a biography. If you are David Lillicrap and would like to personalize this page please email our Author Liaison for assistance. Publications Bispecific Antibody Therapy in Hemophilia The New England Journal of Medicine. Jul, 2017 | Pubmed ID: 28691560 Biological Considerations of Plasma-derived and Recombinant Factor VIII Immunogenicity Blood. Jun, 2017 | Pubmed ID: 28432221 Utility of Factor VIII and Factor VIII to Von Willebrand Factor Ratio in Identifying 277 Unselected Carriers of Hemophilia A American Journal of Hematology. Jun, 2017 | Pubmed ID: 28249352 Summary Report of the First International Conference on Inhibitors in Haemophilia A Blood Transfusion = Trasfusione Del Sangue. Nov, 2016 | Pubmed ID: 27893354 Life in the Shadow of a Dominant Partner: the FVIII-VWF Association and Its Clinical Implications for Hemophilia A Blood. Oct, 2016 | Pubmed ID: 27587878 Functional Characterisation of the Type 1 Von Willebrand Disease Candidate VWF Gene Variants: P.M771I, P.L881R and P.P1413L Blood Transfusion = Trasfusione Del Sangue. Jul, 2016 | Pubmed ID: 27483487 How Much Do We Really Know About Von Willebrand Disease? Current Opinion in Hematology. Sep, 2016 | Pubmed ID: 27428891 Gene Therapy for Coagulation Disorders Circulation Research. Apr, 2016 | Pubmed ID: 27126652 Concurrent Influenza Vaccination Reduces Anti-FVIII Antibody Responses in Murine Hemophilia A Blood. Jun, 2016 | Pubmed ID: 27034428 Clinical and Laboratory Variability in a Cohort of Patients Diagnosed with Type 1 VWD in the United States Blood. May, 2016 | Pubmed ID: 26862110 Recombinant Factor VIII Fc (rFVIIIFc) Fusion Protein Reduces Immunogenicity and Induces Tolerance in Hemophilia A Mice Cellular Immunology. Mar, 2016 | Pubmed ID: 26775174 War and Peace: Factor VIII and the Adaptive Immune Response Cellular Immunology. Mar, 2016 | Pubmed ID: 26676073 To Clear or to Fear: An Innate Perspective on Factor VIII Immunity Cellular Immunology. Mar, 2016 | Pubmed ID: 26547364 Foreword European Journal of Haematology. Dec, 2015 | Pubmed ID: 26679391 Transgene-host Cell Interactions Mediate Significant Influences on the Production, Stability, and Function of Recombinant Canine FVIII Molecular Therapy. Methods & Clinical Development. 2015 | Pubmed ID: 26636112 Journal of Thrombosis and Haemostasis. Special Issue: State of the Art 2015. Preface Journal of Thrombosis and Haemostasis : JTH. Jun, 2015 | Pubmed ID: 26149009 Introduction to a Series of Reviews on Inherited Bleeding Disorders Blood. Mar, 2015 | Pubmed ID: 25712995 Alloantibodies to Therapeutic Factor VIII in Hemophilia A: the Role of Von Willebrand Factor in Regulating Factor VIII Immunogenicity Haematologica. Feb, 2015 | Pubmed ID: 25638804 Rapid Acquisition of Immunologic Tolerance to Factor VIII and Disappearance of Anti-factor VIII IgG4 After Prophylactic Therapy in a Hemophilia A Patient with High-titer Factor VIII Inhibitor Journal of Pediatric Hematology/oncology. May, 2015 | Pubmed ID: 25411864 Syntaxin-binding Protein 5 Exocytosis Regulation: Differential Role in Endothelial Cells and Platelets The Journal of Clinical Investigation. Oct, 2014 | Pubmed ID: 25244090 Analysis of the Role of Von Willebrand Factor, Platelet Glycoprotein VI-, and α2β1-mediated Collagen Binding in Thrombus Formation Blood. Sep, 2014 | Pubmed ID: 25051961 FVIII Stabilization: VWF D'D3 Will Do Blood. Jul, 2014 | Pubmed ID: 25035147 Omental Implantation of BOECs in Hemophilia Dogs Results in Circulating FVIII Antigen and a Complex Immune Response Blood. Jun, 2014 | Pubmed ID: 24829206 A Novel Cell-sheet Technology That Achieves Durable Factor VIII Delivery in a Mouse Model of Hemophilia A PloS One. 2013 | Pubmed ID: 24358271 Von Willebrand Disease: Advances in Pathogenetic Understanding, Diagnosis, and Therapy Hematology. American Society of Hematology. Education Program. 2013 | Pubmed ID: 24319188 Genetic Sequence Analysis of Inherited Bleeding Diseases Blood. Nov, 2013 | Pubmed ID: 24124085 Von Willebrand Disease: Advances in Pathogenetic Understanding, Diagnosis, and Therapy Blood. Nov, 2013 | Pubmed ID: 24065240 Endogenous Factor VIII Synthesis from the Intron 22-inverted F8 Locus May Modulate the Immunogenicity of Replacement Therapy for Hemophilia A Nature Medicine. Oct, 2013 | Pubmed ID: 24037092 The Canadian "National Program for Hemophilia Mutation Testing" Database: a Ten-year Review American Journal of Hematology. Dec, 2013 | Pubmed ID: 23913812 Introduction to a Series of Reviews on Cancer-associated Thrombotic Disease Blood. Sep, 2013 | Pubmed ID: 23798712 The C-type Lectin Receptor CLEC4M Binds, Internalizes, and Clears Von Willebrand Factor and Contributes to the Variation in Plasma Von Willebrand Factor Levels Blood. Jun, 2013 | Pubmed ID: 23529928 Alloantibodies in Von Willebrand Disease Blood. Aug, 2013 | Pubmed ID: 23297130 The Future of Hemostasis Management Pediatric Blood & Cancer. 2013 | Pubmed ID: 23109347 A Complex Substitute: Antibody Therapy for Hemophilia Nature Medicine. Oct, 2012 | Pubmed ID: 23042345 The World Federation of Hemophilia and Research Haemophilia : the Official Journal of the World Federation of Hemophilia. Jul, 2012 | Pubmed ID: 22726078 Von Willebrand Disease: Clinical and Laboratory Lessons Learned from the Large Von Willebrand Disease Studies American Journal of Hematology. Feb, 2012 | Pubmed ID: 22389132 Solulin Increases Clot Stability in Whole Blood from Humans and Dogs with Hemophilia Blood. Apr, 2012 | Pubmed ID: 22234684 The Diagnosis and Management of Von Willebrand Disease in Canada Seminars in Thrombosis and Hemostasis. Jul, 2011 | Pubmed ID: 22102195 Therapeutic Consequences for Misdiagnosis of Type 2N Von Willebrand Disease Pediatric Blood & Cancer. Dec, 2011 | Pubmed ID: 21425451 Pathologic Mechanisms of Type 1 VWD Mutations R1205H and Y1584C Through in Vitro and in Vivo Mouse Models Blood. Apr, 2011 | Pubmed ID: 21346256 Frequency of Platelet Type Versus Type 2B Von Willebrand Disease. An International Registry-based Study Thrombosis and Haemostasis. Mar, 2011 | Pubmed ID: 21301777 A MicroRNA-regulated and GP64-pseudotyped Lentiviral Vector Mediates Stable Expression of FVIII in a Murine Model of Hemophilia A Molecular Therapy : the Journal of the American Society of Gene Therapy. Apr, 2011 | Pubmed ID: 21285959 Early Intraoperative Blood Collection Does Not Affect Complete Blood Counts, Von Willebrand Factor or Factor VIII Levels in Normal Children Journal of Pediatric Hematology/oncology. Jan, 2011 | Pubmed ID: 21178707 Functional Analysis of Three Recombinant A1-VWF Domain Mutants in Comparison to Wild Type and Plasma-derived VWF Facilitates Subtyping in Type 2 Von Willebrand Disease Thrombosis Research. Feb, 2011 | Pubmed ID: 21094983 Challenges and Innovations in the Treatment of Bleeding Disorders Thrombosis Research. Jan, 2011 | Pubmed ID: 21056903 Hierarchical Scaffold Design for Mesenchymal Stem Cell-based Gene Therapy of Hemophilia B Biomaterials. Jan, 2011 | Pubmed ID: 20864158 Eradication of Neutralizing Antibodies to Factor VIII in Canine Hemophilia A After Liver Gene Therapy Blood. Dec, 2010 | Pubmed ID: 20876851 Functional Characterization of a 13-bp Deletion (c.-1522_-1510del13) in the Promoter of the Von Willebrand Factor Gene in Type 1 Von Willebrand Disease Blood. Nov, 2010 | Pubmed ID: 20696945 Improvements in Factor Concentrates Current Opinion in Hematology. Sep, 2010 | Pubmed ID: 20613509 Characterization of Viability and Proliferation of Alginate-poly-L-lysine-alginate Encapsulated Myoblasts Using Flow Cytometry Journal of Biomedical Materials Research. Part B, Applied Biomaterials. Aug, 2010 | Pubmed ID: 20586078 Mutation-specific Hemostatic Variability in Mice Expressing Common Type 2B Von Willebrand Disease Substitutions Blood. Jun, 2010 | Pubmed ID: 20371742 [Application Studies on the Gene Diagnosis and Carrier Detection of Hemophilia A by Using Polymerase Chain Reaction-conformation Sensitive Gel Electrophoresis] Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics. Aug, 2009 | Pubmed ID: 20017302 A Murine Model for Induction of Long-term Immunologic Tolerance to Factor VIII Does Not Require Persistent Detectable Levels of Plasma Factor VIII and Involves Contributions from Foxp3+ T Regulatory Cells Blood. Jul, 2009 | Pubmed ID: 19458355 Recombinant and Plasma-derived Factor VIII Products Induce Distinct Splenic Cytokine Microenvironments in Hemophilia A Mice Blood. Jul, 2009 | Pubmed ID: 19411636 Anti-CD3 Prevents Factor VIII Inhibitor Development in Hemophilia A Mice by a Regulatory CD4+CD25+-dependent Mechanism and by Shifting Cytokine Production to Favor a Th1 Response Blood. Jan, 2009 | Pubmed ID: 18815284 The Role of Molecular Genetics in Diagnosing Von Willebrand Disease Seminars in Thrombosis and Hemostasis. Sep, 2008 | Pubmed ID: 19085649 ADAMTS13 Cleavage Efficiency is Altered by Mutagenic And, to a Lesser Extent, Polymorphic Sequence Changes in the A1 and A2 Domains of Von Willebrand Factor British Journal of Haematology. Nov, 2008 | Pubmed ID: 18986390 Extending Half-life in Coagulation Factors: Where Do We Stand? Thrombosis Research. 2008 | Pubmed ID: 18929522 Coagulation Factors--molecular Attributes and Their Clinical Impact Thrombosis Research. 2008 | Pubmed ID: 18929520 Von Willebrand Disease Pediatric Clinics of North America. Apr, 2008 | Pubmed ID: 18381092 Efficacy and Safety of a New-class Hemostatic Drug Candidate, AV513, in Dogs with Hemophilia A Blood. Jan, 2008 | Pubmed ID: 17978172 Ex Vivo Gene Therapy for Hemophilia A That Enhances Safe Delivery and Sustained in Vivo Factor VIII Expression from Lentivirally Engineered Endothelial Progenitors Stem Cells (Dayton, Ohio). Oct, 2007 | Pubmed ID: 17615271 Distinguishing Between Non-identical Twins: Platelet Type and Type 2B Von Willebrand Disease British Journal of Haematology. Sep, 2007 | Pubmed ID: 17608761 Von Willebrand Disease - Phenotype Versus Genotype: Deficiency Versus Disease Thrombosis Research. 2007 | Pubmed ID: 17490730 Von Willebrand: the Scientist, the Disease, the Factor, and the Treatment Thrombosis Research. 2007 | Pubmed ID: 17482240 Theories of Blood Coagulation Journal of Pediatric Oncology Nursing : Official Journal of the Association of Pediatric Oncology Nurses. May-Jun, 2007 | Pubmed ID: 17475978 Correction of the Bleeding Time in Von Willebrand Factor (VWF)-deficient Mice Using Murine VWF Blood. Mar, 2007 | Pubmed ID: 17312004 The Mutational Spectrum of Type 1 Von Willebrand Disease: Results from a Canadian Cohort Study Blood. Jan, 2007 | Pubmed ID: 17190853 Adenovirus-induced Thrombocytopenia: the Role of Von Willebrand Factor and P-selectin in Mediating Accelerated Platelet Clearance Blood. Apr, 2007 | Pubmed ID: 17148587 The Role of Immunomodulation in the Management of Factor VIII Inhibitors Hematology. American Society of Hematology. Education Program. 2006 | Pubmed ID: 17124093 Genetic Testing for Von Willebrand Disease: the Canadian Experience Seminars in Thrombosis and Hemostasis. Jul, 2006 | Pubmed ID: 16862529 Inhibitor Development in Hemophiliacs: the Roles of Genetic Versus Environmental Factors Seminars in Thrombosis and Hemostasis. Jun, 2006 | Pubmed ID: 16804830 Multiyear Therapeutic Benefit of AAV Serotypes 2, 6, and 8 Delivering Factor VIII to Hemophilia A Mice and Dogs Blood. Jul, 2006 | Pubmed ID: 16522813 Efficient Lentiviral Transduction and Improved Engraftment of Human Bone Marrow Mesenchymal Cells Stem Cells (Dayton, Ohio). Apr, 2006 | Pubmed ID: 16339997 Aminoglycoside Suppression of Nonsense Mutations in Severe Hemophilia Blood. Nov, 2005 | Pubmed ID: 16051741 Identification and Functional Characterization of a Novel 27-bp Deletion in the Macroglycopeptide-coding Region of the GPIBA Gene Resulting in Platelet-type Von Willebrand Disease Blood. Jun, 2005 | Pubmed ID: 15705799 Cell Type-specific Regulation of Von Willebrand Factor Expression by the E4BP4 Transcriptional Repressor Blood. Feb, 2005 | Pubmed ID: 15498853 Heterogeneity of the Immune Response to Adenovirus-mediated Factor VIII Gene Therapy in Different Inbred Hemophilic Mouse Strains The Journal of Gene Medicine. Dec, 2004 | Pubmed ID: 15493040 Molecular Modeling of the Von Willebrand Factor A2 Domain and the Effects of Associated Type 2A Von Willebrand Disease Mutations Journal of Molecular Modeling. Aug, 2004 | Pubmed ID: 15322948 Reliability and Reproducibility of Classification of Children As "bleeders" Versus "non-bleeders" Using a Questionnaire for Significant Mucocutaneous Bleeding Journal of Pediatric Hematology/oncology. Aug, 2004 | Pubmed ID: 15284585 Naked DNA Transfer of Factor VIII Induced Transgene-specific, Species-independent Immune Response in Hemophilia A Mice Molecular Therapy : the Journal of the American Society of Gene Therapy. Jul, 2004 | Pubmed ID: 15233948 A Novel Type 2A Von Willebrand Factor Mutation Located at the Last Nucleotide of Exon 26 (3538G>A) Causes Skipping of 2 Nonadjacent Exons Blood. Nov, 2004 | Pubmed ID: 15226181 Preclinical Animal Models for Hemophilia Gene Therapy: Predictive Value and Limitations Seminars in Thrombosis and Hemostasis. Apr, 2004 | Pubmed ID: 15118932 Helper-dependent Adenoviral Vectors Mediate Therapeutic Factor VIII Expression for Several Months with Minimal Accompanying Toxicity in a Canine Model of Severe Hemophilia A Blood. Feb, 2004 | Pubmed ID: 14512318 Sustained Phenotypic Correction of Canine Hemophilia A Using an Adeno-associated Viral Vector Blood. Sep, 2003 | Pubmed ID: 12738670 Founder Von Willebrand Factor Haplotype Associated with Type 1 Von Willebrand Disease Blood. Jul, 2003 | Pubmed ID: 12649144 Therapeutic Factor VIII Levels and Negligible Toxicity in Mouse and Dog Models of Hemophilia A Following Gene Therapy with High-capacity Adenoviral Vectors Blood. Mar, 2003 | Pubmed ID: 12406898 Gene Expression: Overview and Clinical Implications Vox Sanguinis. Aug, 2002 | Pubmed ID: 12617108 A Survey of Recommendations by Gynecologists in Canada Regarding Oral Contraceptive Use in the Perioperative Period American Journal of Obstetrics and Gynecology. Dec, 2002 | Pubmed ID: 12501060 The Canine Factor VIII 3'-untranslated Region and a Concatemeric Hepatocyte Nuclear Factor 1 Regulatory Element Enhance Factor VIII Transgene Expression in Vivo Human Gene Therapy. Sep, 2002 | Pubmed ID: 12228013 A 4% Solution of Bovine Serum Albumin May Be Used in Place of Factor VIII:C Deficient Plasma in the Control Sample in the Nijmegen Modification of the Bethesda Factor VIII:C Inhibitor Assay Thrombosis and Haemostasis. Aug, 2002 | Pubmed ID: 12195715 Dangerous Liaisons: the Role of "danger" Signals in the Immune Response to Gene Therapy Blood. Aug, 2002 | Pubmed ID: 12149189 Aberrant Splicing and Premature Termination of Transcription of the FVIII Gene As a Cause of Severe Canine Hemophilia A: Similarities with the Intron 22 Inversion Mutation in Human Hemophilia Thrombosis and Haemostasis. Apr, 2002 | Pubmed ID: 12008949 Recombinant Factor IX Recovery and Inhibitor Safety: a Canadian Post-licensure Surveillance Study Thrombosis and Haemostasis. Mar, 2002 | Pubmed ID: 11916075 Genetic Basis of Familial Meniere's Disease The Journal of Otolaryngology. Feb, 2002 | Pubmed ID: 11883436 Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation Alison Michels1, Laura L. Swystun1, Jeffrey Mewburn2, Silvia Albánez1, David Lillicrap1 1Department of Pathology and Molecular Medicine, Queen's University, 2Department of Medicine, Queen's University JoVE 55917 Medicina
Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation Alison Michels1, Laura L. Swystun1, Jeffrey Mewburn2, Silvia Albánez1, David Lillicrap1 1Department of Pathology and Molecular Medicine, Queen's University, 2Department of Medicine, Queen's University JoVE 55917 Medicina